It is featured as the accumulation of pro-inflammatory factors, comprise IL-1β, IL-6, TNF-α and inducible nitric oxide synthase (iNOS) that are generated from activated microglia and astrocytes after ischemic stroke (Jin et al., 2010; DiSabato et al., 2016; Yang and Zhou, 2019). Here, NOS2 is linked to ischemic stroke.