Meanwhile, we performed MS experiments to test the metabolic levels in several tumor tissues (including 8 pairs HLRCC and 6 pairs ccRCC samples), as showed in Fig. 3G–I and Supplementary Fig. S3H–J, it was found that the expression levels of fumarate, glutamine and GSH were significantly up-regulated in FH-deficient RCC compared with ccRCC. The gene discussed is FH; the disease is nonpapillary renal cell carcinoma.