After confirming the successful transfection of the HSPB6 plasmids (Fig. 3D, E), we conducted a series of experiments to understand the function of HSPB6. Firstly, we observed that the proliferation of prostate cancer cells was significantly inhibited after HSPB6 overexpression (Fig. 3F–H), supporting the notion that HSPB6 acts as a tumor suppressor. Here, HSPB6 is linked to prostate carcinoma.