FOXO3 and Familial prostate cancer: Several subclonal copy number alterations were also prominent in the leaf nodes (tips) of the evolutionary tree, e.g. 12q24 LOH in clone C, and 6q LOH in clone G, affecting known prostate cancer driver genes (Intogen) such as NCOR2, FOXO3 and ARID1B. Clone D in particular harboured a loss of FOXP1 (3p13 LOH), a known tumour suppressor gene in prostate cancer [36], and notably, this clone was present in a seminal vesicle sample (LSV).