TDP-43 wild-type (TDP-43WT), cytoplasmic nuclear localization signal mutant (TDP-43ΔNLS), cytoplasmic RNA-recognition motif mutant (TDP-43ΔNLS/4FL), and cytoplasmic mutant (TDP-43ΔNLS/2KQ) that mimics acetylation previously identified in spinal cord tissue of ALS cases50 with carboxy-terminal tagged monomeric GFP (mGFP) were co-expressed with human DNAJB5-FLAG or inactive control (non-fluorescent mutant EGFP51; EGFPY66L-FLAG; herein referred to as CTRL-FLAG). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.