Compared with normal offspring rats, the expression levels of LCII and Beclin-1 in the offspring livers of the GDM group were significantly increased, and the number of autophagosomes in hepatocytes, hepatocyte apoptosis, and hepatocyte edema were also increased; Bcl2, p62, and PPARγ protein expressions were significantly decreased. This evidence concerns the gene SQSTM1 and gestational diabetes.