To further validate CHRNA5’s role with tobacco related HNSCC, we observed the CHRNA5 expression was significantly overexpressed in HNSCC tumors from the larynx when compared to the oral tongue, as most tobacco-related HNSCCs occur in the laryngeal region whereas oral tongue HNSCCs are more associated with HPV positivity(Johnson et al. 2020). Here, CHRNA5 is linked to head and neck squamous cell carcinoma.