BRAF and neoplasm: ddPCR requires minimal DNA input and provides absolute quantification of as little as 3–5 haploid genome equivalents (hGEs) in diagnostic or MRD liquid biopsy samples.18 Successful application of ddPCR for KIAA1549::BRAF detection was demonstrated by Appay et al.19 They implemented a copy number variation (CNV) analysis approach and showed its feasibility using a low concentration of input genomic DNA with a low tumor DNA fraction.