The PI3K/Akt pathway and the MAPK pathway extensively interact in BLCA 40, low expression of HES4 was associated with the MAPK pathway in GSEA analysis, and there was a positive correlation between the expression of HES4 and the IC50 value of AZD6482 in the GDSC database. This evidence concerns the gene AKT1 and bladder transitional cell carcinoma.