An initial automated high throuput drug testing assay using lysosomal inhibitors HCQ and Lys-01, and a Vps34i SAR405 at a wide range of concentrations showed a higher sensitivity of ETV6-RUNX1 cells for HCQ as compared to other pre-B ALL cell lines (Supplementary Table 2 and Supplementary Fig. 2a). Here, ETV6 is linked to acute lymphoblastic leukemia.