In our cohort, the result of univariate analysis showed that high TIM3/Galectin-9 enrichment both on protein (p < 0.001) (Fig. 7c) and transcription level (p < 0.001) (Fig. 7d), age > 60 years (p = 0.045) (Fig. 7e), PS 2–5 (p = 0.036) (Fig. 7f), stage III/IV (p = 0.014) (Fig. 7g), IPI 2–5 (p = 0.005) (Fig. 7h), and LDH > 220 IU/L (p = 0.023) (Fig. 7i) were significant risk factors of OS, and the result of multivariate analysis revealed that high TIM3/Galectin-9 enrichment (p = 0.018) and LDH > 220 IU/L (p = 0.044) were independent prognostic factors for DLBCL patients (Table 2). This evidence concerns the gene LGALS9 and diffuse large B-cell lymphoma.