The generated anti-HER2 antibodies recognize the epitope on both the denatured full-length HER2 protein from the whole cell lysates (SK-BR-3, SK-OV-3) of human cancer cells or recombinant chimeric protein HER2 ECD-Fc (Fig. 2D) and the antigen in native conformation as analyzed on the SK-OV-3 cells (HER2+) by immunofluorescence (IF; Fig. 2E) and ELISA (Fig. 2F). The gene discussed is ERBB2; the disease is cancer.