In a gradient-boosted machine classifier model, prediction of CRS-2 assignment was driven by higher concentrations of lymphotoxin-α, TNF, IFN-α, IFN-γ and IL-15, with additional contributions from mediators reflective of monocyte/macrophage (CCL3, CXCL9) and Th1/Th17 pathway (IL-2, IL-17A, IL-17E, IL-22, IL-1α) activation (Fig. 5e, f). Here, IL22 is linked to craniosynostosis 2.