With stable suppression of GLIPR1 in HGC27 (Fig. 5A), both CAR-T cells increased cytotoxicity against cancer cells (Fig. 4D) and cytokine production of IFN-γ and IL-2 (Fig. 4E and F), suggesting CAR-T cells displayed better functions in gastric cancer with GLIPR1 knockdown. Here, GLIPR1 is linked to gastric cancer.