CHEK2 and neoplasm: To correlate with previous in silico and in vitro results, and to investigate the role of CHEK2 p.I157T and p.E321A variants in tumor malignancy, we evaluated the expression levels of CHK2 in TC tissues and matched adjacent normal tissues from 12 patients (7 with CHEK2 variants, including two cases with p.I157T, two cases with p.E321A, and three cases with p.Y156∗; and 5 without CHEK2 variants) by immunohistochemistry (IHC) staining.