Lastly, regulatory elements of HOTAIR gene were observed to determine temozolomide resistance in U251 glioma cells, possibly via expressional modulation of distant genes, calcium binding, and coiled-coil domain 1 (CALCOCO1) and zinc finger CCCH-type containing 10 (ZC3H10) (Zhang et al. 2020c). The gene discussed is HOTAIR; the disease is central nervous system cancer.