Knockdown of ALKBH5 increased the enrichment of m6A nearby the stop codon and 3’UTR region of CD274 mRNA, thereby promoting the decay of CD274 mRNA in a YTHDF2-dependent manner, but ALKBH5 has minimal inhibitory effects on tumor cell PD-L1 expression at the translational level. The gene discussed is ALKBH5; the disease is neoplasm.