RELA and disease arising from reactivation of latent virus: Two-dimensional gel electrophoresis (2DGE), a system that is well established to monitor alterations in protein charge, revealed migration of RelA towards positive anode during KSHV lytic replication in HOKs and primary lymphatic endothelial cells (LECs), as well as latent infection in Tert-immortalized microvascular endothelial (TIME) cells, (Fig. 1C and S1A).