Similar to the results obtained from PS19 mice, we found no or very weak PGRN immunoreactivity in AT8-positive neurofibrillary tangles (NFTs) and neuritic plaques (NPs) of AD brains, respectively, even though PGRN signal was detected in neurons and significantly increased in dystrophic neurites near amyloid plaques (Fig. 8a and Supplementary Fig. 20b), which is largely consistent with a recent publication76. This evidence concerns the gene GRN and Alzheimer disease.