Proceeding from the hypothesis that the AS-SNP rs2294510 is flagging a genomic region that is more likely to explain the association to the NAFLD phenotype observed in the original GWAS, this study focused on the non-coding region upstream of rs2294510 and of the DIO1 transcription start site (TSS). The gene discussed is DIO1; the disease is metabolic dysfunction-associated steatotic liver disease.