Based on the SHCBP1 expression levels and the purpose of better studying the tumour cell cycle, we mainly used A549 (lowest expression, p53 wild type), NCI-H1299 (medium expression, p53 null), HeLa (highest expression, classic tumour tool cells that are widely used in cell cycle research), and HBE cells in the subsequent experiments. Here, TP53 is linked to neoplasm.