The results of the present study demonstrated that SHCBP1 is another tumour target molecule linked to cell cycle and DNA damage, We investigated the spatial–temporal changes in the nucleoprotein SHCBP1 throughout the tumour cell cycle and revealed that SHCBP1 knockdown induced formation of multipolar spindles because of dysregulated NEK7 and ZW10 expression, and forced premature mitotic entry by significantly down-regulating WEE1­phospho-cdc2 (Tyr15) axis, leading to a compromised G2–M checkpoint. Here, SHCBP1 is linked to neoplasm.