As SHCBP1 played a critical role in cell cycle progression, we then sought to verify whether targeting SHCBP1 could inhibit tumour growth in vitro and vivo. After infection with shSHCBP1 or shCtrl lentivirus, the growth of A549 and HeLa cells were visualized and quantified using the Celigo imaging cytometer for 5 consecutive days. The gene discussed is SHCBP1; the disease is neoplasm.