To test whether the high expression and dynamic localization of SHCBP1 in M phase was closely associated with its functions in tumour cells, we performed SHCBP1 knockdown in NSCLC (A549 and NCI-H1299) and HeLa cell lines to observe their effects on cell cycle progression (Fig. 4A–E). This evidence concerns the gene SHCBP1 and neoplasm.