WEE1 kinase is a key gatekeeper of the G2–M transition through inhibitory phosphorylation of CDK1 at the conserved Tyr15 residues and is an effective anti-cancer target [6, 30–32], we subsequently examined the expression of WEE1 kinase protein and mRNA levels after SHCBP1 knockdown in three tumour cell lines (Fig. 4G, H). Here, CDK1 is linked to neoplasm.