This study identified preoperative comorbidities (diabetes mellitus, coronary artery disease, recent surgery, venous stasis), elevated Platelet (PLT), D-dimer, Ratio of neutrophils to lymphocytes (NLR), Erythrocyte sedimentation rate (ESR), C-reactive protein (CPR), IL-6 (Interleukin-6), Procalcitonin (PCT), reduced red blood cell, advanced age, and AB blood group as high-risk factors for preoperative DVT in TKA patients. The gene discussed is IL6; the disease is diabetes mellitus.