Within this subset, 693 genes are differentially upregulated compared to the other three enterocyte sub-clusters, including the Wnt signaling mediator Ctnnb1, canonical cancer markers STAT3 and HIF1α, and Klf3, Klf4, Klf5 and Klf6, all of which exhibit tumor suppressive properties in many cancers, including CRC [80,90–92] (Figs 3C and S4). This evidence concerns the gene HIF1A and cancer.