Nonetheless, complex structural variations or genetic miscopies (beyond the scope of Sanger sequencing and single nucleotide polymorphism array analysis) might explain this homozygosity in II-1.43 Second, although we demonstrated that heart-specific FARS2 deficiency and FARS2R415L lead to cardiac hypertrophy and HF, additional investigations are needed to ascertain whether the other variants can manifest the HCM phenotypes. This evidence concerns the gene FARS2 and hydrops fetalis.