CASP1 and metabolic dysfunction-associated steatohepatitis: Consistent with findings from previous studies (Wree et al., 2014; Qiu et al., 2018; Koh et al., 2021; Meng et al., 2022), we observed significant pyroptosis, characterised by upregulated protein expression of cleaved-caspase 1 and GSDMD-N, enhanced caspase-1 activity and increased LDH release, in the liver tissue of NASH mice and in mouse primary hepatocytes treated with PA.