Using this multiplexing approach, we simultaneously detected one melanoma (S100), one epithelial (pancytokeratin), 4 blood vessel (AQP1, CD34, αSMA, MECA79), 2 lymphatic vessel (PDPN, LYVE-1), and 4 leukocyte (CD45, CD8, CD20, CD68) markers (Supplementary Table 1). The gene discussed is ACTA1; the disease is melanoma.