C57BL/6 mice were intravenously administered aAVC-PSMA, aAVC-PSA, or aAVC-PAP individually and then inoculated with PSMA-, PSA-, or PAP-expressing melanoma cell lines (B16-PSMA, B16-PSA, and B16-PAP) respectively after 14 days. Here, KLK3 is linked to melanoma.