Using this technique, we were able to detect CIRBP as well as the other biomarkers including cytokines (IL-1β, IL-6, IL-8, MCP-1, and IL-10), markers for endothelial dysfunction (SDC-1, TM, Ang-2, and VEGF), and FGF-23 as a marker for kidney injury in a minimal serum sample volume (25 μl) in all enrolled patients. The gene discussed is VEGFA; the disease is endothelial dysfunction.