SQSTM1 and nonpapillary renal cell carcinoma: To further determine the clinical relevance of VHL-mediated autophagy inhibition, we performed IHC or immunoblotting analyses of human ccRCC specimens with WT-VHL expression and showed that Beclin1 P54-OH levels were inversely correlated with autophagy levels, as reflected by the increased LC3B and decreased p62 expression (Fig. 6A–C).