EPAS1 and nonpapillary renal cell carcinoma: Consistent with a previous publication (Lonergan et al, 1998), ectopical expression of WT Flag-VHL, but not Flag-VHL C162F E3 ligase activity-dead mutant, reduced HIF2α expression (Fig. 1F; Appendix Fig. S1D), which is the primarily expressed HIF isoform in ccRCC (Hoefflin et al, 2020).