There were no significant differences between primary, metastatic, and recurrent samples in the ratio of fibroblasts (number of fibroblasts / number of fibroblasts + cancer cells + immunocytes) (Fig. 1c) and the ratio of α-SMA(+) fibroblasts (number of α-SMA(+) cells / number of fibroblasts) (Fig. 1d). Here, ACTA1 is linked to cancer.