SHC1 and cancer: To validate the correlation between SHC1 and CTNNB1 Ser552, we cultured patient-derived cancer cells (PDCs) from patients of PC-Ra with different expression levels of SHC1 (high-SHC1 group: n = 3, average SHC1 abundance = 9.81; control group: n = 3, average SHC1 abundance = 0.0028) and collected these PDCs to performed LC-MS based phosphoproteomic analysis (Supplementary Fig. 11F).