Considering the anticancer properties of silver nanoparticles and thiosemicarbazide, the current work investigates the anticancer effect of silver nanoparticles functionalized by Glutamine and conjugated with thiosemicarbazide (Ag@Gln-TSC NPs) on a colon cancer cell line and evaluates the expression of the CASPS8, HULC, and PPFIA4 genes in nanoparticle treated cells. The gene discussed is PPFIA4; the disease is colonic neoplasm.