To characterise the ability of the NOMADS16 panel to detect hrp2 and hrp3 deletions that cause false-negative RDT results, we created a set of 45 clonal mock samples with a range of parasitemia levels (625−10,000 parasites per μL) from the lab strains 3D7 (hrp2 + /hrp3 + ), Dd2 (hrp2 − /hrp3 + ) and HB3 (hrp2 + /hrp3 − ). This evidence concerns the gene HDGFL3 and parasitic infectious disease.