To fill this knowledge gap and define the overall roles of excitation and inhibition in STXBP1 encephalopathy pathogenesis, we sought to broadly target glutamatergic neurons by vesicular glutamate transporter 2 (Vglut2)-ires-Cre (Vong et al., 2011) and GABAergic/glycinergic neurons by vesicular inhibitory amino acid transporter (Viaat)-ires-Cre (Vong et al., 2011) since glycine is often co-released with GABA from some inhibitory neurons (Vaaga et al., 2014). The gene discussed is SLC17A6; the disease is Encephalopathy.