Tumor necrosis factor-alpha (TNF-α) rs361525 was significantly associated with decreased risk of osteosarcoma under the heterozygous model (the only model investigated due to unavailability of allele frequency), GA vs GG: OR 0.53 95% CI = 0.28–0.98, P = 0.04. The gene discussed is TNF; the disease is osteosarcoma.