In contrast, EV-A71 replication was markedly reduced in the RD-SCARB2-KO clones (Figs 8D and S9); in a control experiment, susceptibility to infection was restored to KO cells after transfection with a SCARB2 expression plasmid (S10 Fig), eliminating the possibility of off-target effects of CRISPR/Cas9. This evidence concerns the gene SCARB2 and infection.