To confirm the previous identification of human TLR8 as a sensor of major Gram-positive bacteria and therein RNA, we side-by-side applied CQ as pan-endosomal TLR inhibitor and the selective TLR8 inhibitor Cu-Cpt9a (66) to human PBMCs undergoing pneumococcal infection in the absence or presence of TLR2-neutralizing T2.5. This evidence concerns the gene TLR8 and pneumococcal infection.