demonstrated that in the CT26 colorectal cancer model, the addition of anti-VISTA to anti-CTLA-4/anti-PD-1 treatment reduced the expression of genes controlling quiescence, resulting in increased mature cytotoxic CD8+ T-cell subsets, demonstrating that VISTA plays a significant role in maintaining T-cell quiescence (66). This evidence concerns the gene CTLA4 and colorectal cancer.