NOS3 and myocardial ischemia: During myocardial ischemia, the expression (Jeddi et al., 2016[20]) and activity (Giraldez et al., 1997[12]) of the endothelial NOS (eNOS), which is the major contributor to the heart NO production in physiological conditions (Ghasemi and Jeddi, 2022[9]), are diminished, and NO is predominantly produced by the nitrate-nitrite-NO pathway (Samouilov et al., 1998[40]).