Another study has shown that decreased expression of PDX1 gene has a close bearing on the occurrence of GDM and neonatal birth weight change.11,12 As shown in the results of this study, the relative expression of PDX1 in the GDM group was lower than that in the control group (P<0.05), and the gene methylation rate was higher than that in the control group (P<0.05), suggesting a correlation between the decreased expression of PDX1 gene and the increased methylation level and the occurrence of GDM. This evidence concerns the gene PDX1 and gestational diabetes.