The expression of PD-1, PD-L1, and CTLA-4 in the TRG clusters was explored, and the results showed that these three immune checkpoints had higher expression in TRG cluster B than in cluster A. PRDEGs between the two TRG clusters were further determined, and the patients with CRC were split into three gene clusters based on PRDEGs expression. This evidence concerns the gene CTLA4 and colorectal carcinoma.