The expression of key TRGs in normal colon (NCM-460) and CRC (HT-29 and HCT-116) cell lines was validated using qRT-PCR, as shown in Figure 11E. CDK1, BATF, and IL1RN expression was higher in HT-29 and HCT-116 cells than in NCM-460 cells, whereas ITM2A was significantly downregulated in both cell lines (Figure 10E). Here, ITM2A is linked to colorectal carcinoma.