PDX1 and familial pancreatic carcinoma: To characterize MAM heterogeneity in pancreatic cancer liver metastasis in vivo, we induced liver metastasis by intra-portal injection of cells isolated from the genetically engineered ‘KPC’ mouse model of PDAC (KrasG12D; Trp53R172H; Pdx1-Cre), in orthotopic PDAC tumor-bearing mice.