Reg−/− CAR T-cells, co-incubated with isogenic MOLM13-TP53 as well as MV4-11-TP53 AML cells in our established killing assays, dramatically outperformed the untreated and mock CAR T-cell controls both against TP53+/+ as well as TP53−/− AML cells over several E:T ratios and in both MOLM13- and MV4-11-TP53 cells (Fig. 6G,H). This evidence concerns the gene TP53 and acute myeloid leukemia.