We found a significantly higher percentage of acquired CD33 expression on CAR T-cells co-incubated with MOLM13-TP53−/− AML cells than on CAR T-cells co-incubated with MOLM13-TP53+/+ AML cells or the untransduced T-cells in control conditions, hinting at a longer and/or more intimate CAR T-cell:AML cell interaction in the context of TP53 deficiency in AML cells (Fig. 2F,G; Appendix Fig. S3). Here, TP53 is linked to acute myeloid leukemia.