CAR T-cells were co-incubated with isogenic MOLM13-TP53 AML cells with either TP53 wild-type or TP53 null status at an E:T ratio of 1:1 on imaging slides containing microgrids to avoid cell clustering and allow for tracking of individual killing events (Fig. 2H; Movies EV1 and EV2). The gene discussed is TP53; the disease is acute myeloid leukemia.