We did neither observe evidence of antigen-negative escape by outgrowth of CD33− MOLM13-TP53 isogenic AML cells, nor differences in surface expression of the canonical immune checkpoint marker PD-L1 (Programmed cell death 1 ligand 1) at steady state or upon co-incubation with CAR T-cells (Fig. EV1J; Appendix Fig. S4A,B). Here, CD274 is linked to acute myeloid leukemia.