Similarly, in a mouse model of diphtheria and tetanus, when diphtheria and tetanus toxoids (DTaP) antigens were adsorbed into PLGA NPs and co-delivered with a TLR7 ligand, enhanced production of immunoglobulin G (IgG) and IgG2a antibodies was observed, highlighting the potential of PLGA NPs as a potent adjuvants for vaccine formulation [162]. This evidence concerns the gene TLR7 and diphtheria.