In this work, blocking of α7nAChR by MLA in ANI/NEO treated colitis mice, the levels of LC3-II/LC3-I ratio and Beclin-1 were decreased and the P62 was increased, suggeting that α7nAChR mediates the role of ANI/NEO on promoting autophagy in DSS-induced mice. The gene discussed is BECN1; the disease is colitis.