APP and Alzheimer disease: Accordingly, dot-blot quantification of crude tissue lysates of grey matter of this region as well as the associated white matter of AD subjects (n = 17; Braak stage V-VI) and non-AD controls (n = 35; Braak stage 0-IV) were probed for total Aβ (intracellular and extracellular species) via the non-APP cross-reactive MOAB-2 antibody [74] and for tau pathology using the phospho-tau-specific antibody AT-8.