Our study provides solid evidence confirming that loss-of-function variants in TBX20 are clearly associated with disease; according to our data, TBX20tv could explain at least 0.26% of cases with a diagnosis of DCM (isolated or with hypertrabeculation) and 0.75% of isolated LVNC cases. This evidence concerns the gene TBX20 and familial dilated cardiomyopathy.