And in glioma that showed the most outstanding relation between disulfidptosis and immune cell infiltration (Figures 2A, B), its 7-gene signature (risk score = 1.56709174 * APOBEC3C + (-3.2556028) * GLUD1 + (-2.0800874) * KIAA1671 + 1.08729963 * KIF4A + (-7.9141641) * RPL3 + 1.83720741 * TAGLN2 + 1.89252831 * TSPAN31) (Figures 4A–C) was further validated by dividing the TCGA cohort into a training group and a testing group. This evidence concerns the gene RPL3 and glioma.