Through further experiments, it was found that different doses of Wuteng ointment significantly reduced the ROR-γt level in spleen tissue, increased the Foxp3 level, altered the Th17/Treg cell ratio, improved the Th17/Treg cell imbalance in CIA rats, inhibited bone destruction, and played an effective therapeutic role in RA. This evidence concerns the gene FOXP3 and rheumatoid arthritis.