Knockdown of DAP3 and DELE1, respectively, in colon cancer CRC cell lines sensitized the cells to 5-FU, Methotrexate, and Docetaxel, and cells with simultaneous knockdown of DAP3 and DELE1 were markedly sensitive to all the drugs tested, compared with the single knockdown lines and the controls. This evidence concerns the gene DELE1 and malignant colon neoplasm.